Part:BBa_M50433:Design
AtAAE3 coding sequence (yeast optimized) for oxalate catabolism
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 286
Illegal SpeI site found at 526 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 286
Illegal SpeI site found at 526 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 286
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 286
Illegal SpeI site found at 526 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 286
Illegal SpeI site found at 526 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimization was performed on the IDT website (http://idtdna.com/CodonOpt), and a 6x HIS tag was added before the stop codon.
Source
This part was based on the paper "An oxalyl‐CoA synthetase is important for oxalate metabolism in Saccharomyces cerevisiae" by Justin Foster and Paul A. Nakata, published 26 November 2013. The sequence itself was obtained from AEE78480.1 genomic DNA translation on UniprotKB. The actual sequence was submitted by Salanoubat et al.
References
Foster J, Nakata PA 2014. An oxalyl-CoA synthetase is important for oxalate metabolism in Saccharomyces cerevisiae. FEBS Lett. 588(1):160-6.
Salanoubat M, et al. 2000. Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana. Nature. 408(6814):820-2.