Coding

Part:BBa_M50433:Design

Designed by: Sainiteesh Maddineni   Group: Stanford BIOE44 - S11   (2018-04-25)


AtAAE3 coding sequence (yeast optimized) for oxalate catabolism


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 286
    Illegal SpeI site found at 526
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 286
    Illegal SpeI site found at 526
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 286
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 286
    Illegal SpeI site found at 526
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 286
    Illegal SpeI site found at 526
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization was performed on the IDT website (http://idtdna.com/CodonOpt), and a 6x HIS tag was added before the stop codon.


Source

This part was based on the paper "An oxalyl‐CoA synthetase is important for oxalate metabolism in Saccharomyces cerevisiae" by Justin Foster and Paul A. Nakata, published 26 November 2013. The sequence itself was obtained from AEE78480.1 genomic DNA translation on UniprotKB. The actual sequence was submitted by Salanoubat et al.

References

Foster J, Nakata PA 2014. An oxalyl-CoA synthetase is important for oxalate metabolism in Saccharomyces cerevisiae. FEBS Lett. 588(1):160-6.

Salanoubat M, et al. 2000. Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana. Nature. 408(6814):820-2.